Characterization of DNA Polymerase Activity in Trichoplusia ni Cells Infected with Autographa californica Nuclear Polyhedrosis Virus
نویسنده
چکیده
Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral DNA. Based on DNA : DNA hybridization data, virus-specific DNA polymerase activity was insensitive to aphidicolin and novobiocin and was inhibited by ddTTP and N-ethylmaleimide. The data indicate that the AcMNPV-specific DNA polymerase is a ),-like DNA polymerase which was first detected at 5 h postinoculation.
منابع مشابه
Restriction endonuclease analysis to distinguish two closely related nuclear polyhedrosis viruses: Autographa californica MNPV and Trichoplusia ni MNPV.
Restriction endonuclease fragment patterns of the deoxyribonucleic acid genomes of Autographa californica nuclear polyhedrosis virus (multiply embedded type) and Trichoplusia ni nuclear polyhedrosis virus (multiply embedded type) demonstrate that the two viruses are distinct but closely related variants.
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تاریخ انتشار 1987